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A weeks worth of hard work, mentally AND physically, was all worth it! I got so much pure protein of a novel mutation I’ve made to my protein of interest as part of my PhD project! 👩‍🔬 My protein purification protocol is very long and detailed with one of the last steps involving FPLC. My pure protein gets collected in different tubes in a fraction collector. Based on the chromatograph, I decide what fractions to pull and run on gel. I pool together the samples that have the purest samples of
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